Plasmid

Part:BBa_K2813003:Design

Designed by: Matthew Fischer   Group: iGEM18_Michigan   (2018-10-10)


SaCas9 with gRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1255
    Illegal NheI site found at 1278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2305
    Illegal BglII site found at 2458
    Illegal BglII site found at 2604
    Illegal BamHI site found at 1333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1878
    Illegal AgeI site found at 4434
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gRNA has to match the target sequence in order to bind. The scaffold of the gRNA has to match the specific species of Cas9 that you are working with


Source

This part is a nuclease enzyme that is derived from Streptococcus aureus.

References